SAMPLE REQUIREMENTS
Sample quality directly impacts sequencing quality and subsequent bioinformatics analysis. Therefore, NGB Diagnostics has extensive sample quality control procedures to ensure submitted samples conform to requirements for downstream processing.To guarantee the normal processing of your project, samples should meet the standards given below
Notes :
| Library Type | Sample Type | Amount (Qubit) | Volume | Concentration | RNA INTEGRITY NUMBER(AGILENT 2100) | Purity ( NANODROP ™ ) |
|
|---|---|---|---|---|---|---|---|
| Strongly Recommended | Required | ||||||
| Eukaryotic small RNA Sequencing | Total RNA (Animal) | > 6 ug | > 3 ng | > 20 uL | > 50 ng/uL | > 8, smooth base line | No degradation, no contamination |
| Total RNA (Plant and Fungus) |
> 6 ug | > 3 ug | 20 uL | > 50 ng/uL | > 7.5, smooth base line | ||