ribo minus RNA Sequencing

This modification of RNA-Seq is to analyse both Coding and Long Non-Coding (Linc-RNA). The workflow modification is that here from TOTAL RNA, first RIBO depletion is done (NO POLY A enrichment), and straightway taken forward for cDNA conversion and Library preparation.

Sequencing using HiSEQ 4000
2x150 base Read length
40 Million Paired-end Reads
Standard Bioinformatics Included

Standard Transcriptome: mRNA & Whole Transcriptome Bioinformatics:
Quality Control

1. Raw data QC.
2. Removal of low quality reads and trimming of Low quality bases.
3. Adaptor trimming.

Transcriptome Analysis: mRNA & Whole Transcriptome

1. De-Novo Assembly where no reference available.
2. Reference mapping where reference available.
3. Expression/Abundance estimation.
4. Differential Gene expression: MA-Plot, Volcano Plots, heatmaps.
5. Gene Ontology & Pathway analysis.
6. SNP & SSR analysis.
7. Splice variants/isoform analysis and Differential splicing analysis.