RNA Sequencing

NGB diagnostics offers multiple RNA Seq (coding & Non Coding) applications portfolio to help you achieve your Expression based project goals for a reasonable price. Whether you are starting with raw material or need help analyzing data produced elsewhere, let our team of experienced scientists help.

We currently offer multiple sequencing platforms including Illumina HiSEQ4000 & X10. We can generate low to high depth genome reequencing for various applications such as:

1. mRNA Seq- Normal & Stranded mRNA Libraries.
2. Whole transcriptome Sequencing: mRNA +Long Non Coding RNA-seq
3. Small RNA sequencing
4. Degradome Sequencing

mRNA (PolyA) Sequencing

This modification of RNA-Seq is to analyse both Coding and Long Non-Coding (Linc-RNA). The workflow modification is that here from TOTAL RNA, first RIBO depletion is done (NO POLY A enrichment), and straightway taken forward for cDNA conversion and Library preparation.

Normal mRNA Library

Sequencing using HiSEQ 4000
2x150 base Read length
40 Million Paired-end Reads
Standard Bioinformatics Included

Stranded mRNA Library

Sequencing using HiSEQ 4000
2x150 base Read length
40 Million Paired-end Reads
Standard Bioinformatics Included

Standard Transcriptome: mRNA & Whole Transcriptome Bioinformatics:
Quality Control

1. Raw data QC.
2. Removal of low quality reads and trimming of Low quality bases.
3. Adaptor trimming.

Transcriptome Analysis: mRNA & Whole Transcriptome

1. De-Novo Assembly where no reference available.
2. Reference mapping where reference available.
3. Expression/Abundance estimation.
4. Differential Gene expression: MA-Plot, Volcano Plots, heatmaps.
5. Gene Ontology & Pathway analysis.
6. SNP & SSR analysis.
7. Splice variants/isoform analysis and Differential splicing analysis.