Epigenetics Sequencing

SAMPLE REQUIREMENTS

Sample quality directly impacts sequencing quality and subsequent bioinformatics analysis. Therefore, NGB Diagnostics has extensive sample quality control procedures to ensure submitted samples conform to requirements for downstream processing.To guarantee the normal processing of your project, samples should meet the standards given below

Notes :

  • Samples not meeting these specifications should be designated as “at risk” by the customer, and will be subject to billing regardless of data quality. Please consult the Project Manager for further details.
  • Input quantity should be determined by Qubit® instead of by NanoDropTM, and the final quantity and concentration should conform to NGB Diagnostics specifications
Library Type Sample Type Amount (Qubit) Volume Concentration Purity
( NANODROPTM ™ /AGAROSE GEL)
Strongly Recommended Required
ChIP-Seq ChIP-Seq DNA > 100 ng > 50 ng > 10 uL > 20 ng/uL Main peak of 100 bp - 500 bp
Whole Genome Bisulfite Sequencing Genomic DNA > 6 ug > 3 ug > 20 uL > 50 ng/uL No degradation, no contamination