NGB DIAGNOSTICS PRIVATE LIMITED

Pre-prepared library

SAMPLE REQUIREMENTS

Sample quality directly impacts sequencing quality and subsequent bioinformatics analysis. Therefore, NGB Diagnostics has extensive sample quality control procedures to ensure submitted samples conform to requirements for downstream processing.To guarantee the normal processing of your project, samples should meet the standards given below

Notes :

Samples not meeting these specifications should be designated as “at risk” by the customer, and will be subject to billing regardless of data quality. Please consult the Project Manager for further details.
Input quantity should be determined by Qubit® instead of by NanoDropTM, and the final quantity and concentration should conform to NGB Diagnostics specifications

Qualified library standards: qualified insert size, single peak, no miscellaneous peak, no adapters and no primer dimers, Q-PCR verified concentration between 5-30nM.
size: insert + adaptors (120 bp) ± 50 bp (Does not apply to small RNA library). Dilute to 1 ng/µL before checking the insert size by Agilent 2100 Bioanalyzer.
Library concentration: library concentration quantified > 0.5 ng/uL
Molarity concentration: 5 – 30 nM
Library volume requirement:

DATA AMOUNT (X)	DATA AMOUNT (X) VOLUME REQUIREMENT *
X < 30 G	> 10 uL
30 G < X < 100 G	> 20 uL
100 G < X < 400 G	> 30 uL
One flow cell	> 50 uL
Over one flow cell, please contact your technical support.

* High concentration samples should be diluted before delivery

About Us

NGB Diagnostics Pvt. Ltd. founded in the year 2012, is an emerging Indian genomics company, with the mission of facilitating a wide array of Next generation genomic and proteomic services, high-quality molecular biology products, integral solutions for the clinical diagnostics, Pharma, R&D and Agro-food sectors.

Pre-prepared library

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