iTRAQ® (isobaric tags for relative and absolute quantitation)
iTRAQ® is a liquid-based proteomics technique. It labels the Lysine residues and N terminus of all peptides with isotopes of identical masses, thus allows accurate quantitative comparisons between 2 (2-plex) to 8 (8-plex) samples. This approach can provide both protein identification and protein ratios in one experiment.
We have optimized protein extraction for iTRAQ experiment on the following sample types :
